Our manuscript"6-Thioguanine is a noncompetitive and slow binding inhibitor of human deubiquitinating protease USP2" has been published on Scientific Reports (https://www.nature.com/articles/s41598-018-21476-w)! Great job, guys!
Our manuscript "Disulfiram can inhibit MERS and SARS coronavirus papain-like proteases via different modes" has been published on Antiviral Research (https://www.ncbi.nlm.nih.gov/pubmed/29289665)! Congrat, Min-Han, the first author!
Hui-Chi Tang have passed her master defense and graduated this July. Congratulations and good luck, Hui-Chi! New master students, Chi-Chun Lou, Hsin-Chen Lin and Ying Kuan, join our lab. Welcome, freshman!
Our manuscript "Structure of a Highly Active Cephalopod S-crystallin Mutant: New Molecular Evidence for Evolution from an Active Enzyme into Lens-Refractive Protein" has been accepted by Scientific Reports (http://www.nature.com/articles/srep31176)! Great job! Guys!
Cyuan-Ji Chen have passed his master defense and graduated this July. Congratulations and good luck, Cyuan-Ji! New master students, Pei-Rou Jiang and Chih-Hua Hsieh, and one undergraduate, Chin-Hua Yu, join our lab. Welcome, freshman!
Our manuscript "Critical assessment of the important residues involved in the dimerization and catalysis of MERS coronavirus main protease" has been published on PLoS One (http://www.ncbi.nlm.nih.gov/pubmed/26658006)! Great job! Guys!
Shang-Ju Chuang and Bo-Lin Ho have passed their master defense and graduated this July. Congratulations and good luck, boys! New master students, Hui-Chi Tan, Lin Shi and one undergraduate, Hsin-Chen Lin, join our lab. Welcome, freshman!
Our manuscript "Thiopurine analogues and mycophenolic acid synergistically inhibit the papain-like protease of Middle East respiratory syndrome coronavirus" has been published on Antiviral Research (http://www.ncbi.nlm.nih.gov/pubmed/25542975)! Great job! Guys!
The manuscript "Structure and function of a novel single-chain, multi-domain long-chain acyl-CoA carboxylase." has been accepted by Nature! Group of Prof. Liang Tong in Columbia University fills the last piece of jigsaw puzzle of Biotin-dependent carboxylase family. It is a great honor that I have contributed some efforts to the studies. Congratulations!(http://www.ncbi.nlm.nih.gov/pubmed/25383525)
Ting-Yun Wang have passed her master defense and graduated this July. Congratulations and good luck, Ting-Yun! New master student, Cyuan-Ji Chen, and two summer students, Kuan-Yi He and Hsu-Feng Chu, join our lab. Welcome, guys!
Our manuscript for the structural and functional characterization of MERS coronavirus papain-like protease on Journal of Biomedical Science has been qualified as highly accessed! Congratulations! (http://www.jbiomedsci.com/content/21/1/54).
Our manuscript for SARS-CoV papain-like protease has been accepted by Acta Crystallographica Section D: Biological Crystallography (impact factor: 14.1)! This is the first structure of coronaviral PLpro in complex with ubiquitin in the world! Congratulations! (http://www.ncbi.nlm.nih.gov/pubmed/24531491)
Welcome one master student, Po-Lin, and four college students, Min-Han, Yung-Lin, Yi-Cheng and Hau-Tsun join us. Welcome, young guys!
Two of my master students, Yu-Tung Liu and Hung-Yi Chen, have passed their defense and graduated this July. Good luck, Yu-Tung and Hung-Yi!
One more manuscript for SARS-CoV main protease has been accepted by Acta Crystallographica Section D: Biological Crystallography (impact factor: 14.1)! This is our first structure studies using the beam source of Taiwan National Synchrotron Radiation Research Center. The first author is Cheng-Guo Wu. Great jobs, guys! (http://www.ncbi.nlm.nih.gov/pubmed/23633583)
Two of our college students, Shang-Ju and Jing-Wei, have very good performance on their research projects. Thanks their efforts in these years! (http://dls.ym.edu.tw/sa/dls/dls03/dls03-8/newssig.asp?id=31)
The manuscript "Characterizing the importance of the biotin carboxylase domain dimer for Staphylococcus aureus pyruvate carboxylase catalysis" has been accepted by Biochemistry (http://www.ncbi.nlm.nih.gov/pubmed/23286247). The works are the collaboration with Prof. Tong Liang in Columbia University in New York. Congratulations!
Two of my master students, Yi-Hsuan Fang and Ya-Wen Chou, have passed their defense and graduated this July. Good luck, Yi-Hsuan and Ya-Wen!
Prof. Chang has collected numerous chemistry stamps. Recently he is trying to make a digital museum for these valuable treasures (http://www.chemophil.blogspot.tw/). What a wonderful job! Take a look when you guys have time.
Birds leave and come again. One master student, Ting-Yun Wang, joins our group. Wen-Hung, after his Bachelor of Science, will continue his Master learning in our lab. Welcome.
Our manuscript for the folding/unfolding studies on SARS-CoV papain-like protease has been accepted by Archives of Biochemistry and Biophysics (http://www.ncbi.nlm.nih.gov/pubmed/22391227). Congratulations!
One more biotin dependent carboxylase, urea carboxylase, has been solved by our collaborator, prof. Xiang. Congratulations! (http://www.ncbi.nlm.nih.gov/pubmed/22277658)
One Ph.D student, Kevin Cheng, joins our group. Welcome.
The manuscript "Role of a Propeller Loop in the Quaternary Structure and Enzymatic Activity of Prolyl Dipeptidases DPP-IV and DPP9" has been accepted by FEBS Letters (http://www.ncbi.nlm.nih.gov/pubmed/22001206). The works are the collaboration with Dr. Chen Xin in NHRI. Congratulations!
Birds leave and come. Two master students, Yu-Tung Liu and Hung-Yi Chen, and one undergraduate, Jing-Wei Tan, join our group. Welcome.
A new manuscript for E.coli biotin carboxylase has been accepted by Journal of Biological Chemistry (http://www.ncbi.nlm.nih.gov/pubmed/21592965). Most of the structural data were done in Columbia univ. and the kinetic data were in Yang-Ming. Great job!
The collaboration with prof. Tsay Huey-Jen in the institute of neuroscience has a significant paper published on "Neurobiology of Disease" (http://www.ncbi.nlm.nih.gov/pubmed/21220023). By AUC analysis, we measured the oligomerization of beta amyloid peptide, which can be used to explain its neurotoxicity.
This spring, two new young members, Shang-Ju Chuang and Ying-Hsien Lee, join our group. Welcome.
My second master student, Hsing-Yi Lai, has passed his defense and will graduate this January. He will go for the compulsory military service in the near future. Good luck, Hsing-Yi.
A new manuscript for human apolipoprotein E has been accepted by J. Biomed. Sci. (http://www.ncbi.nlm.nih.gov/pubmed/21219628). It spent almost 2 years on paper submission. Finally it reaches.
One more manuscript for development of a AUC technique has been accepted by Methods (http://www.ncbi.nlm.nih.gov/pubmed/21087667). Now AUC can be used to measure the quaternary structure and enzyme kinetic assay simultaneously. It turns on a new application of AUC. This is my 20th papers. Congratulation!
The collaboration between our lab and prof. Chen in Tamkang univ. has good news again. A new manuscript has just been accepted by Toxicol. Mech. Methods (http://www.ncbi.nlm.nih.gov/pubmed/21067310).
My first master student, Shiang-Chuan Chen, has passed his defense and graduated this July. Good luck, Shiang-Chuan.
A new manuscript for zebrafish capsulin protein has been accepted by Protein Expression and Purification! This is the first collaborated paper with Prof. Yau-Hung Chen in Tamkang Univ. Hope the two groups can have more collaborations in the future.
New master students, Ya-Wen Chou and Yi-Hsuan Fang, and summer students, Wei-Hung Chen and Chih-Han Chen, join our group. Welcome!
New manuscript for SARS main protease accepted by Biophysical Journal. Shu-Chun Cheng, Gu-Gang Chang, and Chi-Yuan Chou (2010) "Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease" Biophys. J. 98, 1327-1336. (http://www.cell.com/biophysj/retrieve/pii/S0006349509060858)
We have a new member in the first month of 2010. Juo-Yan Li is my third undergraduate student. Welcome.
Our lab has moved to the 1st floor in Traditional Medical Building A. It is a new start. Changing things, not changing minds.
Welcome Hung-Tien Kang and Cheng-Guo Wu joining our group. Both of them are undergraduate students. Young guys. Welcome.
There are two more persons joining our group. Ms. Li-Chao Chen is the third master student and Ms. Chiung-Wen Pai
is the first Ph.D. student. Welcome
Welcome Hsing-Yi Lai joining our group. He is the second master student.
New review on SARS-CoV papain-like protease accepted by Antiviral Chemistry and Chemotherapy as a Pointer Article. Xin Chen, Chi-Yuan Chou, and Gu-Gang Chang (2009) "Thiopurine analog inhibitors of the SARS-CoV papain-like protease, a deubiquitinating and deISGylating enzyme" Antivir. Chem. Chemother. 19, 151-156.
New manuscript on biotin carboxylase accepted by Journal of Biological Chemistry! Chou Chi-Yuan, Yu Linda P.C., and Tong Liang (2009) "Crystal Structure of Biotin Carboxylase in Complex with Substrates and Implications for Its Catalytic Mechanism" J. Biol. Chem. 284, 11690-11697. (http://www.jbc.org/cgi/content/full/284/17/11690)
Our great professor, Dr. Gu-Gang Chang, is going to retire on February 1st. He taught lots of young scientists in these years and discovered many important mechanisms on enzymology. He also published hundreds of wonderful articles which make a great impact on readers. Lets appreciate his great contribution to science and wish he and his wife can enjoy their retirement with good health!
Welcome Shiang-Chuan Chen joining our group. He is my first master student. Welcome.
Today, I, Chou Chi-Yuan, accept this homepage from the hands of Prof. Chang Gu-Gang. It is a great honor and I will try my best to fit the spirit and goals of molecular enzymology laboratory.
New manuscript on SARS-CoV main protease accepted by Archives of Biochemistry and Biophysics. Lin, P. I., Chou, C. Y., Chang, H. C., Hsu, W. C., and Chang, G. G. (2008) Correlation between dissociation and catalysis of SARS-CoV main protease. Arch. Biochem. Biophys. (in press) doi:10.1016/j.abb.2008.01.023
New manuscript on malic enzyme accepted by Biophys. J. that provides conclusive evidence for the open form II, a molten globule state. Biophys. J. BioFAST: August 17, 2007. doi:10.1529/biophysj.107.111385 [Abstract] [Accepted Manuscript]
Pei-In has won the Poster Award at the 22th Joint Conference of Biomedical Sciences. Congratulations!
Welcome An-Chih Lai and Wei-Kuo Chung joining our group
Chi-Yuan was selected as the recipient of Taiwan Merit Scholarships and will go to the Lab of Professor Tong, Liang at Columbia University (New York). Congratulations! This collaboration already resulted in one review (Current Topics; Biochemistry 2003), two Molecular Cell (2006, 2007) and numerous other papers.
Newly accepted SARS paper from our lab.
Hui-Ping Chang, Chi-Yuan Chou, and Gu-Gang Chang (2007) Reversible Unfolding of the Severe Acute Respiratory Syndrome Coronavirus Main Protease in Guanidinium Chloride. Biophysical Journal (in press) http://www.biophysj.org/cgi/rapidpdf/biophysj.106.091736v1
Dr. Yu-Hou Chen is recruited by Genomic Research Center, Academia Sinica. Congratulations
Welcome Chung, Lo-Hsuan (Nancy) and Lee, Ming-Hsueh joining our lab
Welcome Tsai, Meng-Ying joining our group
Our new home page figure illustrates the application of the rebirth state-of-the-art analytical ultracentrifugation in analysis of protomer interactions in the SARS-CoV main protease.
The sedimentation profiles at various time intervals look like rainbow in the sky. On the ground are interfacial structures, in which the C-terminus and N-terminus symbolize a flower and its root, respectively. We didnot use a colorful spectral rainbow for letting the final art looks like a traditional Chinese painting.
Welcome Dr. Hui-Ping Chang back to our lab with twin babies and tremendous experience.
Welcome Yi-Hui Hsieh joining our group.
Dr. Chi-Yuan Chou passed his final defense for the Ph. D. degree. He is the sixth Ph. D. student training in this lab.
Another SARS main protease paper from our lab was published.
Wen-Chi Hsu, Hui-Chuan Chang, Chi-Yuan Chou, Pui-Jen Tsai, Pei-In Lin, and Gu-Gang Chang (2005) Critical assessment of important regions in the subunit association and catalytic action of the severe acute respiratory syndrome coronavirus main protease. J. Biol. Chem. (in press) (www.jbc.org/cgi/content/abstract/M502556200v1?etoc)
Dr. Hui-Chuan Chang is recruited by the Institute of Biological Chemistry, Academia Sinica. Best wishes to Dr. Chang
As a happy end of 2004, our lab has many things to celebrate. Chi-Yuan just got a daughter, Meng-Ying got admission from Institute of Biopharmaceutical Sciences, and Pei-In got admission from Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Congratulations!
A Commentary of our upcoming ApoE paper (to be published in the Biophys. J.) appears on the web site of the International Atherosclerosis Society (http://www.athero.org/comm-index1.asp#1)
Another manuscript from this lab has been accepted on the day of Moon Festival. Chi-Yuan Chou, Yi-Ling Lin, Yu-Chyi Huang, Sheh-Yi Sheu, Ta-Hsien Lin, Huey-Jen Tsay, Gu-Gang Chang, and Ming-Shi Shiao (2004) Structural Variation in Human Apolipoprotein E3 and E4: Secondary Structure, Tertiary Structure, and Size Distribution. Biophysical Journal (in Press). The reviewer comments it as a fine manuscript that adequately applies modern biophysical analysis methods to studies of apo E3/E4 subdomains.
The first outlet of our SARS research, which is our new area at Yang-Ming, is accepted by the journal Biochemistry.
Chi-Yuan Chou, Hui-Chuan Chang, Wen-Chi Hsu, Tien-Zheng Lin, Chao-Hsiung Lin, and Gu-Gang Chang (2004) Quaternary Structure of the Severe Acute Respiratory Syndome (SARS) Coronavirus Main Protease. Biochemistry 43, 14958-14970. The spinning molecule on this page is the space-filling model of the dimeric SARS CoV main protease.
Dr. Yu-Hou Chen has completed his Ph.D. training in our lab, Congratulations!
Meng-Ying Li was selected by the NSC as the undergraduate student research participant in our lab, Congratulations!
Welcome Teh-Ming Yen, Yi-Hang Lu, Liang-Yu Chen and Pei-Ying Lin join our lab, Welcome!
Dr. Hui-Chuan Chang from our lab as recipient of the NHRI Postdoctoral Fellowship Award, congratulations!
A review article on malic enzyme was accomplished in this lab: Chang, G. G., and Tong, L. (2003) Structure and function of malic enzymes, a new class of oxidative decarboxylases. Biochemistry (in press) (Current Topics) Get the Acrobat Preprint
Welcome back Hsu, Wen-Chi; Welcome Hung, Cheng-Hsiao and Li, Meng-Ying joining our group.
The Molecular Enzymology Laboratory web site was completely revised with AUC section added. The theme figure is the crystal structure of pigeon liver malic enzyme, the first structure solved in this lab in collaboration with Prof. Liang Tong at Columbia U.
We have three new members joining the lab. Yi-Han Li, Yen-Chun Liu from Yang-Ming and a new baby by Hui-Chuan and Zuei-Yen. Congratulations!
The Molecular Enzymology Laboratory is reallocated at National Yang-Ming University, Department of Life Science
Dr. Hui-Chih Hung is recruited by National Chung-Hsing University as Assistant Professor at the Department of Life Science. Congratulations!
Five members of our GROUP visit Columbia University and have GROUP meeting with Professor Liang Tong's GROUP at New York City discussing about the collaborations between two labs
Welcome Professor Liang Tong (Columbia University) visiting Taiwan and our lab
Welcome a little dragon joining the world. Congratulations to Guang-Yaw and Hui-Chih!
Welcome Daniel L. Floyd (from Columbia University) joining our group
Congratulation to Mr. Yu-Hou Chen for passing the qualify examination of the Ph.D. program December, 2000 Congratulations to Dr. Hui-Chih Hung as recipient of the outstanding postdoctor award from the National Health Research Institute
The whole campus of NDMC is moved to Nei-Hu, Taipei. Our new lab web site launched. The structure image of this page is a color version of figure 3 in Biochem. J. 333, 327-334 (1998), representing the active site region of eye lens delta-2 crystallin (argininosuccinate lyase). The figure was generated with RasMol program
Congratulations to Drs. Song-Ling Wang, Jehng-Kang Wang, and Shih-Ming Huang for completing their advanced Ph.D. degree training in the USA
Congratulations to Dr. Hwei-Jen Lee for completing her Ph.D. degree at the Oxford University, UK
Congratulations to Dr. Hui-Chih Hung for completing her Ph.D. degree in our lab
Our Frontiers in Sciences Program "Structure and Function Relationship of Malic Enzyme and Alkaline Phosphatase" was approved by the National Science Council
Our first lab web page launched. The strucure image of that page is a modeled structure of lens S-crystallin (Biophys. J. 76, 679-690, 1999), based on the coordinates of sigma-class glutathione S-transferase.