MEL Lab News

August 28, 2018
Our manuscript "Porcine epidemic diarrhea virus papain-like protease 2 can be noncompetitively inhibitied by 6-thioguanine" has been published on Antiviral Research (! Congrats, Hsu-Feng, the first author!

July 28, 2018
Min-Han Lin has passed his Ph. D defense this July. He is the first Ph. D graduated from my lab. Congrats, Dr. Lin! Also, Pei-Rou Jiang and Chih-Hua Hsieh have passed their master defense. Congrats and good luck, girls! Undergraduate, Chin-Hua Yu and Yung-Shiang Yeh will be master students. Welcome, freshman!

February 15, 2018
Our manuscript"6-Thioguanine is a noncompetitive and slow binding inhibitor of human deubiquitinating protease USP2" has been published on Scientific Reports (! Great job, guys!

December 29, 2017
Our manuscript "Disulfiram can inhibit MERS and SARS coronavirus papain-like proteases via different modes" has been published on Antiviral Research (! Congrat, Min-Han, the first author!

July 13, 2017
Hui-Chi Tang have passed her master defense and graduated this July. Congratulations and good luck, Hui-Chi! New master students, Chi-Chun Lou, Hsin-Chen Lin and Ying Kuan, join our lab. Welcome, freshman!

August 1, 2016
Our manuscript "Structure of a Highly Active Cephalopod S-crystallin Mutant: New Molecular Evidence for Evolution from an Active Enzyme into Lens-Refractive Protein" has been accepted by Scientific Reports (! Great job! Guys!

July 27, 2016
Cyuan-Ji Chen have passed his master defense and graduated this July. Congratulations and good luck, Cyuan-Ji! New master students, Pei-Rou Jiang and Chih-Hua Hsieh, and one undergraduate, Chin-Hua Yu, join our lab. Welcome, freshman!

December 2, 2015
Our manuscript "Critical assessment of the important residues involved in the dimerization and catalysis of MERS coronavirus main protease" has been published on PLoS One (! Great job! Guys!

July 30, 2015
Shang-Ju Chuang and Bo-Lin Ho have passed their master defense and graduated this July. Congratulations and good luck, boys! New master students, Hui-Chi Tan, Lin Shi and one undergraduate, Hsin-Chen Lin, join our lab. Welcome, freshman!

March 1, 2015
Our manuscript "Thiopurine analogues and mycophenolic acid synergistically inhibit the papain-like protease of Middle East respiratory syndrome coronavirus" has been published on Antiviral Research (! Great job! Guys!

September 13, 2014
The manuscript "Structure and function of a novel single-chain, multi-domain long-chain acyl-CoA carboxylase." has been accepted by Nature! Group of Prof. Liang Tong in Columbia University fills the last piece of jigsaw puzzle of Biotin-dependent carboxylase family. It is a great honor that I have contributed some efforts to the studies. Congratulations!(

August 30, 2014
Ting-Yun Wang have passed her master defense and graduated this July. Congratulations and good luck, Ting-Yun! New master student, Cyuan-Ji Chen, and two summer students, Kuan-Yi He and Hsu-Feng Chu, join our lab. Welcome, guys!

June 19, 2014
Our manuscript for the structural and functional characterization of MERS coronavirus papain-like protease on Journal of Biomedical Science has been qualified as highly accessed! Congratulations! (

February 20, 2014
Our manuscript for SARS-CoV papain-like protease has been accepted by Acta Crystallographica Section D: Biological Crystallography (impact factor: 14.1)! This is the first structure of coronaviral PLpro in complex with ubiquitin in the world! Congratulations! (

February 19, 2014
Welcome one master student, Po-Lin, and four college students, Min-Han, Yung-Lin, Yi-Cheng and Hau-Tsun join us. Welcome, young guys!

July 31, 2013
Two of my master students, Yu-Tung Liu and Hung-Yi Chen, have passed their defense and graduated this July. Good luck, Yu-Tung and Hung-Yi!

May 13, 2013
One more manuscript for SARS-CoV main protease has been accepted by Acta Crystallographica Section D: Biological Crystallography (impact factor: 14.1)! This is our first structure studies using the beam source of Taiwan National Synchrotron Radiation Research Center. The first author is Cheng-Guo Wu. Great jobs, guys! (

May 10, 2013
Two of our college students, Shang-Ju and Jing-Wei, have very good performance on their research projects. Thanks their efforts in these years! (

January 7, 2013
The manuscript "Characterizing the importance of the biotin carboxylase domain dimer for Staphylococcus aureus pyruvate carboxylase catalysis" has been accepted by Biochemistry ( The works are the collaboration with Prof. Tong Liang in Columbia University in New York. Congratulations!

July 31, 2012
Two of my master students, Yi-Hsuan Fang and Ya-Wen Chou, have passed their defense and graduated this July. Good luck, Yi-Hsuan and Ya-Wen!

July 27, 2012
Prof. Chang has collected numerous chemistry stamps. Recently he is trying to make a digital museum for these valuable treasures ( What a wonderful job! Take a look when you guys have time.

July 1, 2012
Birds leave and come again. One master student, Ting-Yun Wang, joins our group. Wen-Hung, after his Bachelor of Science, will continue his Master learning in our lab. Welcome.

February 23, 2012
Our manuscript for the folding/unfolding studies on SARS-CoV papain-like protease has been accepted by Archives of Biochemistry and Biophysics ( Congratulations!

January 27, 2012
One more biotin dependent carboxylase, urea carboxylase, has been solved by our collaborator, prof. Xiang. Congratulations! (

November 1, 2011
One Ph.D student, Kevin Cheng, joins our group. Welcome.

October 4, 2011
The manuscript "Role of a Propeller Loop in the Quaternary Structure and Enzymatic Activity of Prolyl Dipeptidases DPP-IV and DPP9" has been accepted by FEBS Letters ( The works are the collaboration with Dr. Chen Xin in NHRI. Congratulations!

August 1, 2011
Birds leave and come. Two master students, Yu-Tung Liu and Hung-Yi Chen, and one undergraduate, Jing-Wei Tan, join our group. Welcome.

May 19, 2011
A new manuscript for E.coli biotin carboxylase has been accepted by Journal of Biological Chemistry ( Most of the structural data were done in Columbia univ. and the kinetic data were in Yang-Ming. Great job!

April 19, 2011
The collaboration with prof. Tsay Huey-Jen in the institute of neuroscience has a significant paper published on "Neurobiology of Disease" ( By AUC analysis, we measured the oligomerization of beta amyloid peptide, which can be used to explain its neurotoxicity.

March 22, 2011
This spring, two new young members, Shang-Ju Chuang and Ying-Hsien Lee, join our group. Welcome.

January 20, 2011
My second master student, Hsing-Yi Lai, has passed his defense and will graduate this January. He will go for the compulsory military service in the near future. Good luck, Hsing-Yi.

December 17, 2010
A new manuscript for human apolipoprotein E has been accepted by J. Biomed. Sci. ( It spent almost 2 years on paper submission. Finally it reaches.

November 10, 2010
One more manuscript for development of a AUC technique has been accepted by Methods ( Now AUC can be used to measure the quaternary structure and enzyme kinetic assay simultaneously. It turns on a new application of AUC. This is my 20th papers. Congratulation!

November 1, 2010
The collaboration between our lab and prof. Chen in Tamkang univ. has good news again. A new manuscript has just been accepted by Toxicol. Mech. Methods (

July 31, 2010
My first master student, Shiang-Chuan Chen, has passed his defense and graduated this July. Good luck, Shiang-Chuan.

July 9, 2010
A new manuscript for zebrafish capsulin protein has been accepted by Protein Expression and Purification! This is the first collaborated paper with Prof. Yau-Hung Chen in Tamkang Univ. Hope the two groups can have more collaborations in the future.

July 1, 2010
New master students, Ya-Wen Chou and Yi-Hsuan Fang, and summer students, Wei-Hung Chen and Chih-Han Chen, join our group. Welcome!

April 7, 2010
New manuscript for SARS main protease accepted by Biophysical Journal. Shu-Chun Cheng, Gu-Gang Chang, and Chi-Yuan Chou (2010) "Mutation of Glu-166 blocks the substrate-induced dimerization of SARS coronavirus main protease" Biophys. J. 98, 1327-1336. (

January 8, 2010
We have a new member in the first month of 2010. Juo-Yan Li is my third undergraduate student. Welcome.

October 17, 2009
Our lab has moved to the 1st floor in Traditional Medical Building A. It is a new start. Changing things, not changing minds.

September 22, 2009
Welcome Hung-Tien Kang and Cheng-Guo Wu joining our group. Both of them are undergraduate students. Young guys. Welcome.

July 6, 2009
There are two more persons joining our group. Ms. Li-Chao Chen is the third master student and Ms. Chiung-Wen Pai is the first Ph.D. student. Welcome

May 15, 2009
Welcome Hsing-Yi Lai joining our group. He is the second master student.

May 1, 2009
New review on SARS-CoV papain-like protease accepted by Antiviral Chemistry and Chemotherapy as a Pointer Article. Xin Chen, Chi-Yuan Chou, and Gu-Gang Chang (2009) "Thiopurine analog inhibitors of the SARS-CoV papain-like protease, a deubiquitinating and deISGylating enzyme" Antivir. Chem. Chemother. 19, 151-156.

February 13, 2009
New manuscript on biotin carboxylase accepted by Journal of Biological Chemistry! Chou Chi-Yuan, Yu Linda P.C., and Tong Liang (2009) "Crystal Structure of Biotin Carboxylase in Complex with Substrates and Implications for Its Catalytic Mechanism" J. Biol. Chem. 284, 11690-11697. (

January 23, 2009
Our great professor, Dr. Gu-Gang Chang, is going to retire on February 1st. He taught lots of young scientists in these years and discovered many important mechanisms on enzymology. He also published hundreds of wonderful articles which make a great impact on readers. Lets appreciate his great contribution to science and wish he and his wife can enjoy their retirement with good health!

December 1, 2008
Welcome Shiang-Chuan Chen joining our group. He is my first master student. Welcome.

July 1, 2008
Today, I, Chou Chi-Yuan, accept this homepage from the hands of Prof. Chang Gu-Gang. It is a great honor and I will try my best to fit the spirit and goals of molecular enzymology laboratory.

February 2, 2008
New manuscript on SARS-CoV main protease accepted by Archives of Biochemistry and Biophysics. Lin, P. I., Chou, C. Y., Chang, H. C., Hsu, W. C., and Chang, G. G. (2008) Correlation between dissociation and catalysis of SARS-CoV main protease. Arch. Biochem. Biophys. (in press) doi:10.1016/

August 10, 2007
New manuscript on malic enzyme accepted by Biophys. J. that provides conclusive evidence for the open form II, a molten globule state. Biophys. J. BioFAST: August 17, 2007. doi:10.1529/biophysj.107.111385 [Abstract] [Accepted Manuscript]

March 29, 2007
Pei-In has won the Poster Award at the 22th Joint Conference of Biomedical Sciences. Congratulations!

February 26, 2007
Welcome An-Chih Lai and Wei-Kuo Chung joining our group

November 26, 2006
Chi-Yuan was selected as the recipient of Taiwan Merit Scholarships and will go to the Lab of Professor Tong, Liang at Columbia University (New York). Congratulations! This collaboration already resulted in one review (Current Topics; Biochemistry 2003), two Molecular Cell (2006, 2007) and numerous other papers.

November 4, 2006
Newly accepted SARS paper from our lab. Hui-Ping Chang, Chi-Yuan Chou, and Gu-Gang Chang (2007) Reversible Unfolding of the Severe Acute Respiratory Syndrome Coronavirus Main Protease in Guanidinium Chloride. Biophysical Journal (in press)

September 12, 2006
Dr. Yu-Hou Chen is recruited by Genomic Research Center, Academia Sinica. Congratulations

September 11, 2006
Welcome Chung, Lo-Hsuan (Nancy) and Lee, Ming-Hsueh joining our lab

August 13, 2006
Welcome Tsai, Meng-Ying joining our group

February 22, 2006
Our new home page figure illustrates the application of the rebirth state-of-the-art analytical ultracentrifugation in analysis of protomer interactions in the SARS-CoV main protease. Art design: The sedimentation profiles at various time intervals look like rainbow in the sky. On the ground are interfacial structures, in which the C-terminus and N-terminus symbolize a flower and its root, respectively. We didnot use a colorful spectral rainbow for letting the final art looks like a traditional Chinese painting.

November 8, 2005
Welcome Dr. Hui-Ping Chang back to our lab with twin babies and tremendous experience.

September 1, 2005
Welcome Yi-Hui Hsieh joining our group.

June 5, 2005
Dr. Chi-Yuan Chou passed his final defense for the Ph. D. degree. He is the sixth Ph. D. student training in this lab.

April 16, 2005
Another SARS main protease paper from our lab was published. Wen-Chi Hsu, Hui-Chuan Chang, Chi-Yuan Chou, Pui-Jen Tsai, Pei-In Lin, and Gu-Gang Chang (2005) Critical assessment of important regions in the subunit association and catalytic action of the severe acute respiratory syndrome coronavirus main protease. J. Biol. Chem. (in press) (

March 3, 2005
Dr. Hui-Chuan Chang is recruited by the Institute of Biological Chemistry, Academia Sinica. Best wishes to Dr. Chang

December 29, 2004
As a happy end of 2004, our lab has many things to celebrate. Chi-Yuan just got a daughter, Meng-Ying got admission from Institute of Biopharmaceutical Sciences, and Pei-In got admission from Institute of Biochemistry and Molecular Biology, National Yang-Ming University, Congratulations!

December 4, 2004
A Commentary of our upcoming ApoE paper (to be published in the Biophys. J.) appears on the web site of the International Atherosclerosis Society (

September 28, 2004
Another manuscript from this lab has been accepted on the day of Moon Festival. Chi-Yuan Chou, Yi-Ling Lin, Yu-Chyi Huang, Sheh-Yi Sheu, Ta-Hsien Lin, Huey-Jen Tsay, Gu-Gang Chang, and Ming-Shi Shiao (2004) Structural Variation in Human Apolipoprotein E3 and E4: Secondary Structure, Tertiary Structure, and Size Distribution. Biophysical Journal (in Press). The reviewer comments it as a fine manuscript that adequately applies modern biophysical analysis methods to studies of apo E3/E4 subdomains.

September 15, 2004
The first outlet of our SARS research, which is our new area at Yang-Ming, is accepted by the journal Biochemistry. Chi-Yuan Chou, Hui-Chuan Chang, Wen-Chi Hsu, Tien-Zheng Lin, Chao-Hsiung Lin, and Gu-Gang Chang (2004) Quaternary Structure of the Severe Acute Respiratory Syndome (SARS) Coronavirus Main Protease. Biochemistry 43, 14958-14970. The spinning molecule on this page is the space-filling model of the dimeric SARS CoV main protease.

June 27, 2004
Dr. Yu-Hou Chen has completed his Ph.D. training in our lab, Congratulations! Meng-Ying Li was selected by the NSC as the undergraduate student research participant in our lab, Congratulations! Welcome Teh-Ming Yen, Yi-Hang Lu, Liang-Yu Chen and Pei-Ying Lin join our lab, Welcome!

April 3, 2004
Dr. Hui-Chuan Chang from our lab as recipient of the NHRI Postdoctoral Fellowship Award, congratulations!

October 2, 2003
A review article on malic enzyme was accomplished in this lab: Chang, G. G., and Tong, L. (2003) Structure and function of malic enzymes, a new class of oxidative decarboxylases. Biochemistry (in press) (Current Topics) Get the Acrobat Preprint

September 30, 2003
Welcome back Hsu, Wen-Chi; Welcome Hung, Cheng-Hsiao and Li, Meng-Ying joining our group.

December 21, 2002
The Molecular Enzymology Laboratory web site was completely revised with AUC section added. The theme figure is the crystal structure of pigeon liver malic enzyme, the first structure solved in this lab in collaboration with Prof. Liang Tong at Columbia U.

November, 2002
We have three new members joining the lab. Yi-Han Li, Yen-Chun Liu from Yang-Ming and a new baby by Hui-Chuan and Zuei-Yen. Congratulations!

August, 2002
The Molecular Enzymology Laboratory is reallocated at National Yang-Ming University, Department of Life Science

February, 2002
Dr. Hui-Chih Hung is recruited by National Chung-Hsing University as Assistant Professor at the Department of Life Science. Congratulations!

June 1, 2001
Five members of our GROUP visit Columbia University and have GROUP meeting with Professor Liang Tong's GROUP at New York City discussing about the collaborations between two labs

May, 2001
Welcome Professor Liang Tong (Columbia University) visiting Taiwan and our lab

January 21, 2001
Welcome a little dragon joining the world. Congratulations to Guang-Yaw and Hui-Chih!

January 12, 2001
Welcome Daniel L. Floyd (from Columbia University) joining our group

January, 2001
Congratulation to Mr. Yu-Hou Chen for passing the qualify examination of the Ph.D. program December, 2000 Congratulations to Dr. Hui-Chih Hung as recipient of the outstanding postdoctor award from the National Health Research Institute

February 7, 2000
The whole campus of NDMC is moved to Nei-Hu, Taipei. Our new lab web site launched. The structure image of this page is a color version of figure 3 in Biochem. J. 333, 327-334 (1998), representing the active site region of eye lens delta-2 crystallin (argininosuccinate lyase). The figure was generated with RasMol program

Congratulations to Drs. Song-Ling Wang, Jehng-Kang Wang, and Shih-Ming Huang for completing their advanced Ph.D. degree training in the USA

August 2, 1999
Congratulations to Dr. Hwei-Jen Lee for completing her Ph.D. degree at the Oxford University, UK

July, 1999
Congratulations to Dr. Hui-Chih Hung for completing her Ph.D. degree in our lab

March 6, 1998
Our Frontiers in Sciences Program "Structure and Function Relationship of Malic Enzyme and Alkaline Phosphatase" was approved by the National Science Council

September 3, 1997
Our first lab web page launched. The strucure image of that page is a modeled structure of lens S-crystallin (Biophys. J. 76, 679-690, 1999), based on the coordinates of sigma-class glutathione S-transferase.

Last Revised September 11 2018 04:00:36.